, 1998). This effect co-exists with highly irregular firing on a single-cell level. Our findings allow for making testable predictions and can

be linked to cortical substrates of memory ABT-199 clinical trial function. We examined oscillatory and spiking phenomena emerging during simulated memory retrieval in two different paradigms using a layer 2/3 attractor network. The network had a hypercolumnar structure (Fig. 1) spanning some 1.5×1.5 mm2 of a subsampled cortical sheet and comprising ~15,000 Hodgkin–Huxley-type multi-compartmental neurons and ~2,000,000 synapses. The model was constituted by 9 hypercolumns each containing 49 minicolumns. Pyramidal cells within the same functional minicolumn had dense recurrent connections and common inputs from layer 4 (Yoshimura et al., 2005). Each hypercolumn was defined by the minicolumns

sharing non-specific feedback inhibition (Yoshimura et al., 2005) from the same basket cell pool, and thus extending ~500 μm (Yuan et al., 2011). The model operated Akt targets in a bistable regime (Amit and Brunel, 1997, Djurfeldt et al., 2008 and Lundqvist et al., 2010) with two distinct network states. During a so-called non-coding ground state all pyramidal cells exhibited low-level irregular activity (~0.2 s−1, Cv2=0.97±0.20), whereas in the coding attractor state each hypercolumn acted as a winner-take-all module with cells in only one minicolumn active at an elevated rate (~3–10 s−1, Cv2=0.98±0.25). There were 49 distinct, globally distributed patterns of network activity, or cell assemblies, acting as attractor memories. Although these patterns ( Fig. 1) were set up manually (see Experimental procedures), they could be assumed to have been formed by prior learning. They consisted of subsets of minicolumns, one from every hypercolumn, connected by structured horizontal

long-range axons ( Muir et al., 2010). The cell assemblies had finite life-time Glutathione peroxidase due to the mechanism of cellular adaptation (see Experimental procedures), which forced them to terminate after ~300 ms and caused the network to return to the ground state, i.e. its default operational mode. In this work we considered two alternative approaches to disrupting this default state dynamics and forcing the network’s transition to the coding attractor state. They relate to two separate memory phenomena but result in similar retrieval dynamics once a cell assembly activation is initiated. The first approach, functionally corresponding to pattern completion from a fragmentary input, consisted in partial stimulation of one of the stored memory patterns (stimulation of 5 out of 9 minicolumns participating in a unique distributed pattern, see Experimental procedures) leading to a short-lasting activation of the cell assembly (Fig. 2A). In every 20-s simulation, 20 different patterns were stimulated (partially cued) at a rate of 1 s−1.