Anal Biochem 1983,
132:259–264.Angiogenesis inhibitor CrossRefPubMed 31. Clarkson JJ: International collaborative research on fluoride. J Dent Res 2000, 79:893–904.CrossRef 32. Cross SE, Kreth J, Zhu L, Sullivan R, Shi W, Qi F, Gimzewski JK: Nanomechanical properties of glucans and associated cell-surface adhesion of Streptococcus mutans probed by atomic force microscopy under in situ conditions. Microbiology 2007, 153:3124–3132.CrossRefPubMed 33. Dibdin GH, Shellis RP: Physical and biochemical studies of Streptococcus mutans sediments suggest new factors linking the cariogenicity of plaque with its extracellular polysaccharide content. J Dent Res 1988, 67:890–895.CrossRefPubMed 34. Kreth J, Zhu L, Merritt J, Shi W, Qi F: Role of sucrose in the fitness of Streptococcus mutans. Oral Microbiol Immunol 2008, 23:213–219.CrossRefPubMed see more 35. Yamashita Y, Bowen WH, Burne RA, Kuramitsu HK: Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model. Infect Immun 1993, 61:3811–3817.PubMed 36. Paes Leme AF, Koo H, Bellato CM, Bedi G, Cury JA: The role of sucrose in cariogenic dental biofilm formation–new
insight. J Dent Res 2006, 85:878–887.CrossRefPubMed 37. Vacca-Smith AM, Scott-Anne K, Whelehan MT, Berkowitz RJ, Feng C, Bowen WH: Salivary glucosyltransferase B as a possible marker for caries activity. Caries Res 2007, 41:445–450.CrossRefPubMed 38. Griswold AR, Jameson-Lee M, Burne RA: Regulation and physiologic significance of the agmatine deiminase system of
Streptococcus mutans UA159. J Bacteriol 2006, 188:834–841.CrossRefPubMed 39. Loesche WJ, Henry CA: Intracellular microbial polysaccharide EPZ004777 production and dental caries in a Guatemalan Indian Village. Arch Oral Biol 1967, 12:189–194.CrossRefPubMed 40. Spatafora G, Rohrer K, Barnard D, Michalek S: A Streptococcus mutans mutant that synthesizes elevated levels of intracellular polysaccharide is hypercariogenic in vivo. Infect Immun 1995, 63:2556–2563.PubMed 41. Tanzer JM, Freedman ML, Woodiel FN, Eifert RL, Rinehimer LA: Association of Streptococcus mutans virulence with synthesis of intracellular polysaccharide. Proceedings in microbiology. Aspects of dental caries. Special Amrubicin supplement to Microbiology Abstracts (Edited by: Stiles HM, Loesche WJ, O’Brien TL). London: Information Retrieval, Inc 1976, 3:596–616. Authors’ contributions JGJ planed and carried out the biofilm experiments and the biochemical assays, and also assisted with the data analysis and drafted the manuscript. MIK carried out all the molecular genetic studies and collected, organized and analyzed the real-time PCR data. JX conducted all the LSCFM studies, including image acquisition, data collection and analysis. PLR organized the data, helped to draft the manuscript and revised it for important intellectual content. HK conceived the study, participated in its design and coordination, and was involved in drafting the manuscript and revising it critically for intellectual content.