“
“Objective: Although stem cells hold a great therapeutic potential for injured tissues, limited survival of transplanted stem cells has hindered the clinical application of this technology. We hypothesized that an omentum-based stem cell-supporting patch could provide adequate nutrients and microenvironment to prolong cell survival. We examined this hypothesis in rats with experimental myocardial infarction.

Methods: The omentum-based supporting patch was constructed by stitching polylactic acid-co-glycolic acid polymer seeded with mesenchymal stem cells from male Sprague-Dawley

rats. Eight weeks after the experimental myocardial infarction, which was created by ligating the left coronary artery of female Sprague-Dawley

rats, c-Met inhibitor mesenchymal stem cells were transplanted with (n = 16) or without (n = 14) the supporting patch. After 4 weeks, transplanted mesenchymal stem cell survival, ventricular remodeling, and cardiac performance were examined.

Results: Significantly more cells survived after 4 weeks in rats transplanted with mesenchymal stem cells on the supporting patch assessed by means of polymerase chain reaction detection of the Sry gene than seen in those without the supporting patch (2.61 +/- 0.40 vs 1.19 GSK461364 +/- 0.12, P<.05). Rats with myocardial infarction that received mesenchymal stem cells with the patch also had significantly improved ventricular remodeling and cardiac function than those without the patch. Wrapping infarcted myocardium with omentum alone did not change the myocardial function.

Conclusions: The omentum-based cell-supporting patch provided a favorable microenvironment for transplanted mesenchymal stem cell survival, which resulted in favorable ventricular remodeling https://www.selleck.cn/products/mek162.html and

restoration of cardiac function in rats with experimental myocardial infarction. Further validation of the technique in human subjects could make mesenchymal stem cell transplantation a viable therapeutic option for patients with cardiac disease. (J Thorac Cardiovasc Surg 2010;140:1388-96)”
“Thyroid hormones (THs) play a crucial role in the maturation and functioning of mammalian central nervous system. Thyroxine (T4) and 3, 3′, 5-L-triiodothyronine (T3) are well known for their genomic effects, but recently attention has been focused on their non genomic actions as modulators of neuronal activity. In the present study we report that 14 and 13 reduce, in a non competitive manner, GABA-evoked currents in rat hippocampal cultures with IC(50)s of 13 +/- 4 mu M and 12 +/- 3 mu M, respectively. The genomically inactive compound rev-T3 was also able to inhibit the currents elicited by GABA. Blocking PKC or PKA activity, chelating intracellular calcium, or antagonizing the integrin receptor alpha V beta 3 with TETRAC did not affect THs modulation of GABA-evoked currents. THs affect also synaptic activity in hippocampal and cortical cultured neurons.