“
“OBJECTIVE: To develop a reliable preimplantation genetic diagnosis protocol for antihuman platelet antigen-1 incompatibility for a family in whom antenatal treatment was not possible because of the mother’s hypersensitivity to intravenous
immunoglobulin (IVIG).
METHODS: Haplotypes were constructed from genomic DNA of the family members. A polymerase chain reaction protocol that included eight microsatellite polymorphic markers and the ITGB3-specific (T196C, rs5918) polymorphism were multiplexed to be used in a single cell protocol, and single blastomeres were analyzed.
RESULTS: In one preimplantation genetic diagnosis cycle, out of 28 retrieved oocytes, 24 embryos fertilized and 12 underwent biopsy. Three embryos were found to be antihuman platelet antigen-1b/1b homozygotes and two were Temsirolimus in vivo transferred. This cycle resulted in an uneventful pregnancy and birth of a healthy child.
CONCLUSION: In cases in which there is antihuman platelet antigen
incompatibility and IVIG cannot be administered, preimplantation genetic diagnosis is a reliable alternative Alvocidib clinical trial to enable birth of unaffected children. (Obstet Gynecol 2012;119:338-43) DOI: 10.1097/AOG.0b013e318242a11d”
“Introduction: Cancer-related carbohydrate epitopes, which are regarded as potential diagnostic and prognostic biomarkers, are carried on the main acute phase proteins. It is not clear, however, if the glycosylation profile is similar in different glycoproteins, or it is protein specific to some extent. The aim of the study was to compare fucosylation, alpha 2,3 sialylation and expression of sialyl-Lewis(x) epitopes (sLe(x)) in the serum as a whole, AGP and haptoglobin of small cell (SCLC) and non-small cell lung cancer (NSCLC) patients with respect to healthy subjects
as well as the cancer stage and its histological type.
Material and Methods: Thirty-three NSCLC, 13 SCLC patients and 20 healthy volunteers were included AZD1208 in the study. Carbohydrate epitopes were detected by means of their reactivity with specific lectins and monoclonal anti-sLe(x) antibodies in direct or dual-ligand ELISA tests.
Results: Significantly increased fucosylation was found in total serum in both cancer groups and in NSCLC haptoglobin. No difference was observed in SCLC haptoglobin or alpha(1)-acid glycoprotein in both cancer groups. Also alpha 2,3 sialylation was elevated in total serum, but not in alpha(1)-acid glycoprotein. This type of sialylation was undetectable in haptoglobin by means of MAA reactivity, in both healthy and cancer subjects. Complete sLe(x) antigens were overexpressed in total NSCLC serum and SCLC AGP, and their level was considerably lowered in cancer haptoglobin.
Discussion: Typical acute phase proteins, haptoglobin and AGP, exhibit different glycosylation profiles in lung cancer. Alterations observed in haptoglobin reflected the disease process better than those in AGP.