2b), the role of the genetic background was highlighted. In some cases, the same agricultural practice in combination with the same soy variety, the outcome was a close
grouping (e.g., for conventional Legend 2375). However, a third sample of the same Legend 2375, also grown under a conventional practice showed an intermediate distance to the mentioned samples, but grouped very closely to an organic sample of Legend 2375. For other pairs of varieties grown under the same agricultural practice, samples grouped with an intermediate distance (GM Stine 2032 and conventional Asgrow 2869), yet other pairs showed a great distance between sample characteristics (organic ED4315, organic Pioneer 9305). Soy from the three different categories, GM, conventional buy Panobinostat and organic, could be well separated (Fig 3). The first axis of variation BMN673 mainly separated organic
samples from both the GM and conventional, while the second axis differentiated the GM from conventional. GM soybeans were most strongly associated with saturated and mono-unsaturated fatty acids. Organic soybeans were associated with elements and amino acids Zn, Asp, Lys, Ala, Sr, Ba, Glu. Conventional soy were associated with the elements Mo and Cd (Fig. 4). The model accounted for 21.5% of the total variation in the material (PC1 = 19.0%, PC2 = 2.5%). Our data demonstrate that different agricultural practices lead to markedly different end products, i.e., rejecting the null
hypothesis (H0) of substantial equivalence between the three SPTLC1 management systems of herbicide tolerant GM, conventional and organic agriculture. Both the H1 and H2 hypotheses were supported due to the key results of high levels of glyphosate/AMPA residues in GM-soybeans, and that all the individual soy samples could be discriminated statistically (without exception) into their respective agricultural practice background – based on their measured compositional characteristics (Fig. 3). Notably, the multivariate analyses of the compositional results was performed excluding the factors glyphosate/AMPA residues, which obviously otherwise would have served as a strong grouping variable separating the GM soy from the two non-GM soy types. Since different varieties of soy (different genetic backgrounds) from different fields (environments) grown using different agricultural practices were analysed, we need to acknowledge that variation in composition will come from all three of these sources. However, since 13 samples out of the 31 had at least one ‘sibling’ (same variety) to compare both within and across the different agricultural practices, how the same variety ‘performed’ (i.e., its nutritional and elemental composition) between different environments and agricultural practices could be compared. As some samples of the same variety were highly similar in the cluster analysis, but others were intermediate or even highly different (Fig.