The effect of palm oil content on liposome formation, surface morphology, shape, size and zeta potential of the liposomes were studied using scanning electron microscopy (SEM) transmission electron microscopy (TEM) and particle analyzer. Drug loading, entrapment efficiency and in vitro drug release were measured in phosphate-buffered saline (PBS, pH 7.4) by UV spectrophotometry.
Results TEM and SEM images showed formation find protocol of liposomes for all formulations, However, increase in the proportion of palm oil in the formulations significantly reduced particle size and increased zeta potential. The effect on drug loading and drug release varied with palm oil fraction. The best release pattern with appropriate entrapment
efficiency and stability was obtained with liposomes containing 33 % palm oil fraction. Introduction of 46 and 56 % of palm oil fractions yielded zeta potential of -42.8 and -50.7 mV, respectively, compared with -31.2 mV for the formulation without palm oil.
Conclusion: The results demonstrate the potentials of palm oil fractions in the preparation of suitable DS liposomes with good bioavailability.”
“The effects of sodium butyrate (SB), a histone deacetylase inhibitor, in combination with cisplatin (CDDP), for inhibition of cell growth and induction of apoptosis were investigated in bladder cancer cell lines in vitro. Bladder cancer cell lines T24,
253J, and UMUC3 were treated with different concentrations of CDDP or SB. Cell proliferation was studied by XTT assay. Cell-cycle analysis and Selleckchem ATR inhibitor induction of apoptosis click here were analyzed by laser scanning cytometry (LSC). Western blot analysis was used to determine expression of p21, p27, TRADD, FADD, caspase-2, and caspase-7. We observed that SB in combination with CDDP induced significant inhibition of cell growth in a dose-dependent manner through G(1) arrest and apoptosis, as determined by LSC. When bladder cancer cell lines were treated with SB plus CDDP, Western blotting showed increased expression
of p21 but not p27 in T24 cells, whereas both p21 and p27 increased in 253J and UMUC3 cells. All cell lines exhibited a moderate increase in TRADD and decrease in procaspase-2 but no significant change in FADD and procaspase-7. The results showed the synergistic anticancer effect of SB in combination with CDDP, their potential for treatment of bladder cancer, and their mechanism of action in terms of cell signal transduction and induction of apoptosis.”
“The aim of this study was to determine the prevalence of thyroid dysfunction and its association with cardiovascular risk factors in an adult Bulgarian population. 2402 subjects were studied, 1347 female, 20-94y (median: 48.0y) and 1055 male, 20-91y (median: 45.5y). Body weight, height, waist circumference, arterial blood pressure, TSH, FT4 and lipids were measured. Known hypothyroidism was reported by 53 subjects (2.2%) and hyperthyroidism by 20 (0.8%).